Download A Guide to Human Gene Therapy by Roland W. Herzog, Sergei Zolotukhin PDF

By Roland W. Herzog, Sergei Zolotukhin

ISBN-10: 9814280909

ISBN-13: 9789814280907

Ever because the start of molecular biology, the tantalizing risk of treating disorder at its genetic roots has turn into more and more possible. Gene remedy although nonetheless in its infancy is still one of many most well-liked parts of study in medication. Its method makes use of a gene move car (''vector'') to carry healing DNA or RNA to cells of the physique as a way to rectify the disorder that's inflicting the sickness. winning remedies were pronounced in people lately reminiscent of remedies in boys with critical immune deficiencies. in addition, gene treatment recommendations are being tailored in several biomedical laboratories to acquire novel remedies for numerous illnesses and to review uncomplicated organic features of affliction. Correction of illness in animal experiences, is progressively gaining flooring, highlighting the great capability of gene remedy within the clinical occupation.

This publication will disguise issues which are on the leading edge of biomedical study comparable to RNA interference, viral and non-viral gene move platforms, remedy of hematological illnesses and problems of the crucial frightened procedure. major specialists at the respective vector or disorder will give a contribution the person chapters and clarify state of the art applied sciences. It additionally offers a wide assessment of an important gene move vectors and so much largely studied goal illnesses. This finished advisor is as a result a must-read for an individual within the biotechnology, biomedical or scientific industries trying to extra their wisdom within the region of human gene treatment

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Extra resources for A Guide to Human Gene Therapy

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Lipofection: a highly efficient, lipid-mediated DNAtransfection procedure. Proc Natl Acad Sci USA 84: 7413–7417. 17 May 4, 2010 11:0 SPI-B903 9in x 6in b903-ch01 A Guide to Human Gene Therapy 4. Felgner PL, Ringold GM (1989). Cationic liposome-mediated transfection. Nature 337: 387–388. 5. Wolff JA, et al. (1990). Direct gene transfer into mouse muscle in vivo. Science 247: 1465–1468. 6. Nabel GJ, et al. (1996). Immune response in human melanoma after transfer of an allogeneic class I major histocompatibility complex gene with DNA-liposome complexes.

40. Bedikian AY, Del Vecchio M (2008). Allovectin-7 therapy in metastatic melanoma. Expert Opin Biol Ther 8: 839–844. 41. Reyes L, et al. (2001). Vaxfectin enhances antigen specific antibody titers and maintains Th1 type immune responses to plasmid DNA immunization. Vaccine 19: 3778–3786. 42. Hartikka J, et al. (2001). Vaxfectin enhances the humoral immune response to plasmid DNA-encoded antigens. Vaccine 19: 1911–1923. 43. Wu CH, Wilson JM, Wu GY (1989). Targeting genes: delivery and persistent expression of a foreign gene driven by mammalian regulatory elements in vivo.

In vitro and in vivo transfection of plasmid DNA in the Dunning prostate tumor R3327-AT1 is enhanced by focused ultrasound. Gene Ther 7: 1516–1525. 29. Greenleaf WJ, Bolander ME, Sarkar G, Goldring MB, Greenleaf JF (1998). Artificial cavitation nuclei significantly enhance acoustically induced cell transfection. Ultrasound Med Biol 24: 587–595. 30. Takeuchi D, et al. (2008). Alleviation of Abeta-induced cognitive impairment by ultrasound-mediated gene transfer of HGF in a mouse model. Gene Ther 15: 561–571.

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