Download Advanced Methods in Cellular Immunology by Rafael Fernandez-Botran PDF

By Rafael Fernandez-Botran

ISBN-10: 0849321255

ISBN-13: 9780849321252

Immunologists in addition investigators in different disciplines might frequently use protocols regarding the isolation, cultures and characterization of alternative different types of leukocytes. complicated tools in mobile Immunology is a suite of suggestions in an easy-to-use format.Each bankruptcy offers readers with similar application details, a step by step description of the method, substitute innovations, pertinent references, and data approximately advertisement assets for fabrics and regents. as well as leukocytes, the authors advisor readers throughout the methods of telephone tradition in addition to irritation and autoimmunity in numerous animal models.Covering subject matters corresponding to PCR and Apoptosis, this e-book will serve a advisor to universal systems in mobile immunology whereas using either human and murine versions.

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J. and Cohn, Z. , Purification and cultivation of monocytes and macrophages, in In vitro Reagents in Cell-Mediated and Tumor Immunity, Bloom, B. A. and Davis, J. , Academic Press, San Diego, 1976, 333. 11. Ackerman, S. K. and Douglas, S. , Purification of human monocytes on microexudate-coated surfaces, J. , 120, 1372, 1978. 12. , Wagenaar-Hilbers, J. P. , and Wauben, M. H. , Adjuvant arthritis in the rat, in Current Protocols in Immunology, Coligan, J. , Kruisbeek, A. , Margulies, D. , Shevach, E.

Immunol. , 212, 99, 1998. 5. , Bartal, A. , An enzymelinked immunosorbent assay (ELISA) for the detection of monoclonal antibodies recognizing antigens expressed on viable cells, J. Immunol. Reagents, 58, 301, 1983. 6. , DNA staining for fluorescence and laser confocal microscopy, J. Histochem. , 45, 49, 1997. 7. Denizot, F. , Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability, J. Immunol. Reagents, 89, 271, 1986.

100 g/ml. Dilute the stock Percoll with HBSS to create solutions of the desired densities. 8. Prepare a multiple discontinuous density gradient. 090 g/ml), underlayer with the solutions of increasing density using flexible, 21-gauge tubing connected to a syringe. Use a 15-ml conical centrifuge tube. 9. Carefully layer the granulocyte suspension on top of the gradient. 10. Centrifuge at 700 × g for 20 min. 1% gelatin solution in HBSS. 11. 5 ml of ice-cold water for 30 s. 5 ml of a 10× HBSS (Ca2+ and Mg2+ free).

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