By Kevan M. A. Gartland, Michael R. Davey
Agrobacterium Protocols bargains starting and skilled researchers the main entire choice of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the subjects variety from the upkeep of bacterial tradition collections to features of the metabolism and body structure of remodeled tissues and transgenic vegetation. Drawing at the paintings of top scientists from laboratories worldwide, Agrobacterium Protocols offers a wealth of innovations for introducing particular DNA sequences into aim plant species and discusses the environmental implications of genetically engineered vegetation. Its special techniques will facilitate quick move of complex thoughts to different laboratories and their exploitation in basic and utilized plant biology.
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Additional info for Agrobacterium Protocols (Methods in Molecular Biology Vol 44)
Therefore, the antisense approach has been widely used to study the functional role of a gene and to eliminate or reduce undesirable characteristics from important crops. Transgenic tomato expressing antisense RNA of l-aminocyclopropane-1-carboxylate (ACC) synthase, inhibits fruit ripening (24). Ripening of a fruit can be induced by ethyl- Binary Ti Plasmid HrndIII pGA580 55 Vectors XbUI SSII Kpnt HiI BglII CIaI A AGC TTC TAG AGC TCG TTA ACG GTA CCA TCG ATA GAT CT HmdIII xbd SSII BgrII pGA58 1 AA GC T TCT AGA GCT CG T TCG ATA GAT CT HmdIII pGA582 SSII KpnI HP1 BglII AAG CTT CTA GAG CTC GTT AAC GGT ACC ATC GCG ATA GAT CT HtndIII pGA809 XbaI SCUI MIUI Ml01 EcoRl ClaI BglII AGC TTC AGT ACT ACG CGT CTC GAG AAT TCA TCG ATA GAT CT Fig.
Plant Mol. Biol. 19,15-38. , and Schell, J. (1983) Ti plasmrd vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacrty. EMBO J 2,2143-2150. 5. , Murphy, E. B , Boberts, J L , Gustafson, G. , and Malcolm, S. K (1985) Resistance to hygromycin B A new marker for plant transformation studres. Plant Mol Biol 5, 103-108. 6 Holt, J. , Powles, S. , and Holtum, A M. (1993) Mechanisms and agronomic aspect of herbicide resistance. Annu. Rev. Plant Physiol.
In some strains of A. tumefaciens, exposure to these inducing conditions leads to inhibition of bacterial growth, which can then be followed by the selection of avirulent mutants (I). Observation of this effect of vir gene induction depends on the use of particular experimental conditions. This chapter first specifies experimental parameters allowing to reveal, in A. tumefaciens, the modifications that accompany vir gene induction. Other parts of the chapter then describe how the mutants that are generated in the presence of acetosyringone are recognized and characterized.